group rows based on a string in a column in pandas and count the number of occurrence of unique rows that contained the string

I have a dataset with a few columns. I would like to slice the data frame with finding a string "M22" in the column "Run number". I am able to do so. However, I would like to count the number of unique rows that contained the string "M22".

Here is what I have done for the below table (example):

RUN_NUMBER  DATE_TIME   CULTURE_DAY AGE_HRS AGE_DAYS
335991M 6/30/2022   0   0   0
M220621 7/1/2022    1   24  1
M220678 7/2/2022    2   48  2
510091M 7/3/2022    3   72  3
M220500 7/4/2022    4   96  4
335991M 7/5/2022    5   120 5
M220621 7/6/2022    6   144 6
M220678 7/7/2022    7   168 7
335991M 7/8/2022    8   192 8
M220621 7/9/2022    9   216 9
M220678 7/10/2022   10  240 10

here is the results I got:

RUN_NUMBER
335991M      0
510091M      0
335992M      0
M220621      3
M220678      3
M220500      1

Now I need to count the strings/rows that contained "M22" : so I need to get 3 as output.

source https://stackoverflow.com/questions/75379009/group-rows-based-on-a-string-in-a-column-in-pandas-and-count-the-number-of-occur

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